Grafted superporous cellulose bead for protein chromatography
Glycidyl methacrylate (GMA) was grafted onto the pore surface in superporous cellulose (SC) beads using ceric ammonium (CAN) nitrate as initiator. The grafted and ungrafted matrices were modified with diethylaminoehtyl (DEAE) groups. The effects of initiator concentration and reaction time on the grafting polymerization were studied to find a proper grafting condition. Infrared spectral analysis confirmed the grafting reaction. The adsorption capacity of bovine serum albumin (BSA) to the DEAE-G- SC was over two times increased as compared to the ungrafted DEAE-SC. Due to the high adsorption capacities of the grafted beads, the DEAE-G-SC column could be operated at a DBC of 20％ to 35％ higher than the normal DEAE-SC column in the range of 150 to 600 cm/h. Hence, it is considered that grafting polymer chains to superporous beads promises to improve the adsorption capacity of chromatographic stationary phases.
Grafting polymerization Superporous cellulose bead Anion exchanger Protein adsorption High capacity
Dong-Mei Wang Yan Sun
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
国际会议
The 5th International Conference on Separation Science and Technology(第五届国际分离科学与技术会议)
北京
英文
2007-10-14（万方平台首次上网日期，不代表论文的发表时间）