Displacement chromatography of proteins on hydrophobic charge induction adsorbent column

摘要：

Displacement chromatography of protein mixtures was proposed on a hydrophobic charge induction chromatography (HCIC) column. We have used an HCIC medium, MEP Hypercel as the stationary phase and a quaternary ammonium salt, benzethonium chloride, as the displacer. The capacity factors of proteins and displacers were used to predict their performances in column displacement, and the experimental results agreed well with the prediction. An isotachic displacement train of lysozyme and |á -chymotrypsinogen A was formed with benzethonium chloride as displacer at pH 5.0 with good yields and purities of the two proteins. Column regeneration was efficiently achieved by charge repulsion between the displacer and the adsorbent at lower pH values. The results indicate that the displacement chromatography on HCIC is a good alternative to traditional hydrophobic displacement chromatography.

关键词： displacement chromatography proteins hydrophobic charge induction chromatography(HCIC) MEP-Hypercel lysozyme α -chymotrypsinogen A

作者: Guofeng Zhao Yan Sun

作者单位: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

会议类型: 国际会议

会议名称: The 5th International Conference on Separation Science and Technology(第五届国际分离科学与技术会议)

会议地点: 北京

会议语种:英文

在线出版日期: 2007-10-14（万方平台首次上网日期，不代表论文的发表时间）