Metabolic modeling of mutated Saccharomyces cerevisiae producing 1,2-propanediol
1,2-propanediol cannot be produced by wild type Saccharomyces cerevisiae, but can be produced by gene manipulation of which methylglyoxal reductase(mgs) and glycerol dehydrogenase(gldA) genes are imported to Saccharomyces cerevisiae interior. The branching point that produce 1,2-propanediol in the Saccharomyces cerevisiae is dihydroxyacetone phosphate which is the intermediate of glycolysis pathway. In this study we observed the effect of enzymatic reaction steps in mutated Saccharomyces cerevisiae producing 1,2-propanediol. In order to investigate the effect of enzymatic reaction steps in 1,2-propanediol metabolism in Saccharomyces cerevisiae, we have constructed a metabolic network model of mutated strain of Saccharomyces cerevisiae by using available information from literatures databases. In the model, the each reaction step on the full scale metabolic network of Saccharomyces cerevisiae was expressed in a mathematical form. For simulation research, we observed glycolysis and 1,2-propanediol branch ratio by changing each reaction flux and concentration of metabolites in this network. In the results, we found that optimized condition of metabolic network producing 1,2-propanediol. From the results of this mathematical expression and modeling work, we could establish a new mutated Saccharomyces cerevisiae strain which can produce high yield of 1,2-propanediol. It can be applied to the analysis of 1,2-propanediol metabolism and increase the yield of 1,2- propanediol by Saccharomyces cerevisiae.
1,2-propanediol Metabolic Analysis Saccharomyces creivisiae
Se In Kim Jinwon Lee
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742,Republic of Ko Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742,Republic of Ko
国际会议
The 5th International Conference on Separation Science and Technology(第五届国际分离科学与技术会议)
北京
英文
2007-10-14(万方平台首次上网日期,不代表论文的发表时间)