Array-based pyrosequencing
With the completion draft of the human genome, we are entering a new era in the biological sciences with the DNA sequencing as one of the main catalysts. High throughput and affordable DNA sequencing techniques would undoubtedly continue the stream of the revolution initiated by the Human Genome Project. Recent impressive advances in DNA sequencing technologies have accelerated the detailed analysis of genomes from many organisms. We have been observing numerous reports of complete or draft versions of the genome sequence of several well-studied, multi-cellular organisms. The Human Genome Project was made achievable by a significant reduction in DNA sequencing cost by three orders of magnitude; a further cost reduction of two to three would launch a new era of DNA sequencing applications from short DNA reads to whole genome sequencing. In the DNA sequencing field, Pyrosequencing has emerged as a technology for de novo high throughput whole genome sequencing. The method is based on the principle of sequencing-by-synthesis and pyrophosphate detection through a series of enzymatic reactions to generate luminescence sequence peak signals. Pyrosequencing is being used from single nucleotide to genome sequencing and the method is commercially available for low throughput sequencing by Biotage and high throughput sequencing by 454 Life Sciences (currently owned by Roche). Further developments and improvements could increase the throughput and decrease the cost of sequencing by another two orders of magnitude within the next five years.
Baback Gharizadeh Sanket Goel Helmy Eltoukhy Mohsen Nemat-Gorgani Ali Agah Farbod Babrzadeh Roxana Jalili Amir-Ali H.Talasaz Ronald W.Davis Mostafa Ronaghi
Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）