SINGLE CELL SAMPLING AND ANALYSIS BY ULTRA SHORT PULSE LASER

摘要：

In order to obtain molecular information on single-cell basis, two new techniques which utilized ultra short pulse laser were developed. The first technique was the laser micro-scalpel for biological samples, which enables precise dissection of heat labile and/or water embedded samples. Using this technique, the highly farmable tissue of Eucommia plant and water embedded zebrafish embryo were successfully dissected. The second technique was novel non-liner four wave mixing microscope named stimulated parametric emission (SPE) microscopy. This technique was applied to observe cell components of the non-stained living tobacco BY-2 cells.

关键词： femto-second Laser micro-scalpel single-cell sampling stimulated parametric emission microscopy

作者: Shinichiro KAJIYAMA Masaaki SAKAKURA Mitsuyoshi UEDA Kazuyoshi ITOH Kiichi FUKUI

作者单位: Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University 2-1 International Innovation Center of Kyoto University, Kyotodaigaku-Katsura Nishikyo-ku, Kyoto 615-852 Graduate school of Agruculture, Kyoto University, Kitashirakawa Oiwake-cho Sakyo-ku, Kyoto 606-8502

会议类型: 国际会议

会议名称: The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)

会议地点: 苏州

会议语种:英文

在线出版日期: 2007-09-10（万方平台首次上网日期，不代表论文的发表时间）