Automated High-throughput SNP Detection using Magnetic Composite Particles
(0) A high-throughput automated approach for single nucleotide polymorphisms (SNPs) genotyping using solid-phase PCR on magnetic composite particles (MCPs) was developed. Biotin-labeled reverse primer was captured by streptavidin magnetic composite particles (SA-MCPs), PCR products were directly amplified on the surface of SA-MCPs in a 96-well plate. The ssDNA amplified on MCPs was hybridized with a pair of dual-color probes, and then genotype of each sample can be simultaneously identified by scanning the microarray printed with the denatured fluorescent probes. All the procedures were performed on an automated workstation equipped with a magnetic separation device. The M235T polymorphism in angiotensinogen (AGT) gene from 160 samples was successfully interrogated by this method. With automated operation and microarray based “read-out technology, this approach will be a novel, simple and labor-saving method for large-scale SNP detection.
magnetic composite particles solid-phase PCR SNP genotyping automated workstation
Nongyue He Song Li Hongna Liu
State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering.Southeast University, Nanjing, 210096, China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）