Application of Genechip in S-genotyping of Pear Cultivars
(0) Pear is an important fruit tree with abundant varieties and rich genetic resources. It has large cultivated area in the world. Pear displays gametophtic self-incompatibility (GSI) and its self-incompatibility genotype (S-genotype) is useful for arrangement of pollination varieties in commercial orchard as well as breeding programs. Several approaches for S-genotyping of cultivars have been developed such as crossing experiments, PCR-RLFP system, DNA sequence analysis and cDNA cloning etc. In this paper, the advantages and disadvantages of these methods were analyzed, and a new genechip-based method was put forward. According to the structural features of pear S-RNase gene, some oligonucleotide probes were designed and successfully applied in assay of the S-genotypes of pear cultivars, which provides foundation for pear S-genotyping with the method of S-RNase genechip.
pear self-incompatibility S-RNase S-genotyes genechip
Xiao-Feng Tan Nan Jiang Lin Zhang Yan-Ling Zeng Dang-Quan Zhang Hong Cheng
The Key Lab.of Non-wood Forest Product of State Forestry Administration, Central South University of Key Laboratory of Green Packaging and Biological Nanotechnology, Hunan University of Technology, Zhu
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）