Microarray based Raman spectroscopic detection with gold nanoparticle probes
In this paper, we developed a novel microarray based surface-enhanced Raman spectroscopic (SERS) assay for detection of peptide and protein interaction by attachment of gold nanoparticles followed by silver deposition for signal enhancement. The attachment of the gold nanoparticles is achieved by standard avidin-biotin chemistry. The well-known biomolecular recognition systems, IgG with protein A and biotin with avidin, were chosen here to establish the SERS assay. In the spectroscopic assay, peptide is detected down to a limit of 10 fg on the microarray spots. And recognition of standard proteins (protein A and IgG) down to 0.1 mg/mL for proteins in solution and 100 fg for proteins on the microarray spots are also demonstrated.
Microarray SERS Protein interaction Gold nanoparticles
Tao Li Liping Guo Zhenxin Wang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chine School of Chemistry, Northeast Normal University, Changchun 130022, China State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chine
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）