AFM Imaging of the Interaction between Fe2O3 Nanoparticles and Human Liver Cancer Cells

摘要：

We report a strategy for rapid and low cost DNA sequencing method in which the sequences of DNA templates, hybridized to a surface-immobilized array of DNA primers, are determined by sensing the number of the extended fluorescence labeled nucleotides. The primers in the array are extended in sequential DNA polymerase-catalyzed nucleotide incorporation reactions with a low concentration single fluorescein-labeled deoxyribonucleoside triphosphate (dNTP), and followed by filling the vacancy with the corresponding unlabeled dNTP. We have sussessfully sequenced 12 bp DNA template with this method. The method has potential for the rapid and low-cost DNA sequencing with microarray platform.

关键词： DNA re-sequencing SBS DNA microarray fluorescein-labeled dNTP

作者: Shuchun Li Yanyan Wang Ningping Huang Yu Zhang Haitao Wang Zhongdang Xiao

作者单位: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, SiPaiLou 2#, Nanjing 210096 P.R.China

会议类型: 国际会议

会议名称: The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)

会议地点: 苏州

会议语种:英文

在线出版日期: 2007-09-10（万方平台首次上网日期，不代表论文的发表时间）