Cellular Interaction base on PEG-mediated Fused Cells On the RT-CESTM System
A novel method was developed to prepare functionalized magnetic nanoparticles (MNPs), in which SiO2/(PMMA/Fe3O4) magnetic nanoparticles (MNPs) were modified with 3-aminopropyl triethoxysilane to produce a dense amine surface. The amido modified MNPs (NH2-MNPs) were capable of efficient electrostatic capture of genomic DNA. The maximum amount of genomic DNA captured on 10 μg of NH2-MNPs was about 700 ng. PB buffer effectively released the captured DNA. This efficiency was dramatically enhanced by incubation at 80 ℃ and DNA recovery from NH2-MNPs approached 90％. The NH2-MNPs were employed to simplify the isolation of genomic DNA from human whole blood. It is a promising method for high-throughput automated extraction of genomic DNA from whole blood samples at a decreased yield.
magnetic nanoparticles human whole blood genomic DNA
Mengdie Qian Xuan Liu Dingxin Zhou Zaozao Chen Zuming Tang Huihua Deng Zuhong Lu
Key Laboratory of Child Development and Learning Science of Ministry of Education, Southeast Univers Chien-Shiung Wu Laboratory, School of Biological Science and Medical Engineering, Southeast Universi Key Laboratory of Child Development and Learning Science of Ministry of Education, Southeast Univers
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）