Determination of hepatoma-specific alpha-fetoprotein by a mini-column affinity chromatography method
Cellular interaction and adhesion is a dynamic and integrated process requiring the participation of specialized cell surface receptors, structural proteins, signaling proteins, and the cellular cytoskeleton. In this paper, we choose a label-free and real-time method to measure and monitor cell adhesion on special microplates integrated with electronic cell sensor arrays. These plates were used in conjunction with the real-time cell electronic sensing (RT-CES?) system to dynamically and quantitatively monitor the specific interaction of cells. We do some treatment on them 24h after plant. It can be conclude that the system is sensitive enough to finger out some little changes of cells. In order to find out the mechanism of membrane fusion of cells, we use PEG(poly ethylene glycol) to make non-small lung cancer cells (A549 cells) fuse and plant them back into microwells on RT-CES, we can also get the changes from the plot of the experiment.
cellular interaction RT-CES PEG cell fusion
Wei Wu Deng-Fu Yao Li-Wei Qiu Xin-Hua Wu
Research Center of Clinical Molecular Biology, Affiliated Hospital, Nantong University, Nantong 226001, China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）