Effect of Zinc Oxide Nanoparticles in Different Size under UV Irradiation on SMMC-7721 Cancer Cells

摘要：

We demonstrated the first use of X-ray photoelectron spectroscopy (XPS), a relatively sensitive and less time-dependent technique to study the hybridization of DNA. The target DNA strand labeled with chlorine-containing Hexachloro-Fluorescein (HEX) was hybridized to DNA arrays with four different probes. Every probe dot of the arrays was detected with XPS. The XPS Cl2p peak area was found to decrease with the increase of mismatched base numbers in DNA probes. The XPS Cl2p peak area ratio for perfectly matched probe to one base mismatched probe, two bases mismatched probe and three bases mismatched probe accorded well with the corresponding fluorescent intensity ratio from fluorescent measurements, evidencing that XPS is a potential tool for the analysis of DNA hybridization.

关键词： DNA array Hybridization X-ray photoelectron spectroscopy

作者: Da-dong Guo Chun-hui Wu Xue-mei Wang

作者单位: State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, China.

会议类型: 国际会议

会议名称: The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)

会议地点: 苏州

会议语种:英文

在线出版日期: 2007-09-10（万方平台首次上网日期，不代表论文的发表时间）