Gold nanoparticle-based detection of transcription factor NF-κB
Transcription factor and sequence specific DNA interaction plays important role in drug genome and transcription therapy. Gold nanoparticles show high sensitivity, stability and compatibility for biological molecules as electrochemical intercalators. There is no report of the detection of sequence specific DNA binding transcription factor by gold nanoparticle catalyzed-Ag enhancement at interface between electrodes and electrolyte solutions. Here unimolecular hairpin oligonucleotides were self-assembled onto Au electrode surface and elongation on solid phase was carried out to double strand oligonucleotides with transcription factor NF-κB binding sites. Gold nanoparticle-catalyzed Ag deposition was detected by anodic stripping voltammetry (ASV) for NF-κB binding. It was indicated that the method for sequence specific DNA binding protein detection showed pronounced specificity, sensitivity and gave highlight for transcription regulation, operator site and functional gene inspection.
NF-κB gold nanoparticle Ag enhancement anodic stripping voltammetry
Qin Pan Nongyue He Yunfei Bai Renyun Zhang Zuhong Lu
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096，China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）