An Improved DNA Sequencing-by-synthesis Method

摘要：

This paper reported an improved sequencing-by-synthesis method. The read length was 19 bases for the sequence with a hairpin structure immobilized onto the surface of aldehyde glass slides. The Cy5-labeled deoxyribonucleoside triphosphate(dNTP) species were incorporated in the extension reactions and the fluorescein label was destroyed by hydrogen peroxide(H2O2) after the readout. The optimal conditions for the fluorescence quenching and the mechanism of reaction were explored. The extension efficiency in the single step was about 80％ after quenching of the fluorescence. The improved sequencing-by-synthesis method could sequence more than 80 bases in principle. The images of fluorescence collected from the array during the extension process were reanalyzed with the prepared program of Matlab software.

关键词： Sequencing-by-synthesis Fluorescence quenching H2O2 Matlab

作者: Li Gao Hua Lu Zhengying Qian Zuhong Lu

作者单位: State Key Laboratory of Bioelectronics, Southeast University,Nanjing,210096,China

会议类型: 国际会议

会议名称: The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)

会议地点: 苏州

会议语种:英文

在线出版日期: 2007-09-10（万方平台首次上网日期，不代表论文的发表时间）