A pollen-specific calmodulin-binding protein from rice is essential to pollen development
A pollen-expressed gene of rice that encodes a novel calmodulin (CaM)-binding protein named OsPCBP was previously identified. The role of QsPCBP in pollen germination and tube growth has been determined. To further investigate the function of OsPCBP in pollen development, we generated transgenic plants using RNA interference (RNAi) approach. Analysis of the OsPCBP–RNAi plants found that each produced approximately equal amounts of normal and aborted pollen. Microscopic examination of the microspores at different stages of anther development revealed that microspores of the OsPCBP–RNAi plants developed normally until the binucleate stage. However, about half of the microspores were aborted at subsequent stages. RT-PCR and protein gel blot analysis of the aborted pollen grains showed that the deletion of OsPCBP was correlated with pollen abortion phenotype. Our experimental results provide convincing evidence that OsPCBP plays an important role in pollen development, especially for the later stage of pollen development.
pollen calmodulin-binding protein RNA interference rice
Qiusheng Zhang Zhong Li Jing Yang Shaoqin Li Yangsheng Li Daichang Yang Yingguo Zhu
College of Life Science, Ludong University, Yantai, Shandong, 264025, China Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, W College of Life Science, Ludong University, Yantai, Shandong, 264025, China; Key Laboratory of MOE f
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）