Differential expression of proteins of human ovaries from normal and polycystic ovarian syndrome by 2-dimensional gel electrophoresis/mass spectrometry analysis
Polycystic ovary syndrome (PCOS) is the most common cause for anovulatory infertility, affecting 5-10％ of females of reproductive age. Currently, little is known about the changes in whole proteins between PCOS and normal ovaries. In the present study, a proteomic approach comprised of two-dimensional gel electrophoresis (2DE) analysis and mass spectroscopy was used to identify proteins and examine expression patterns in three PCOS and normal ovaries, respectively. One hundred and ten protein spots were separated and showed different intensities between PCOS and normal ovaries. Sixty-nine proteins associated with cellular metabolism and physiological process were identified from 72 spots. Fifty-four proteins were up-regulated in PCOS ovaries and 15 other proteins were up-regulated in normal ovaries. These data demonstrate for the first time the complexity in the regulation of ovarian protein expression in human PCOS, and will provide important insight for a better understanding of the pathogenetic mechanisms underlying this clinical disorder.
2-dimensional gel electrophoresis mass spectrometry ovary PCOS
Xiang Ma Lu Fan Yan Meng Zheng Hou Yun-Dong Mao Wei Wang Wei Ding Jia-Yin Liu
Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, P.R.China;The Cente Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, P.R.China The Center of Clinical Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical Unive
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）