Effect of Inhibition of DLC-1 Gene Expression by RNA Interference (RNAi) on Cell Proliferation and Migration in Colon Cancer LoVo Cell Line
DLC-1 (deleted in liver cancer 1) is a potential tumor suppressor gene, which is inactive in liver carcinogenesis. To observe the effect of DLC-1 gene expression on cell proliferation and migration in human colon cancer cell line, RNAi Lipo-recombinent of DLC-1 gene(pGCsiDLC)were constructed and transduced into LoVo cells which are positive for DLC-1 gene expression. The results have shown that RNAi recombinent could effectively inhibit the expression of DLC-1 gene in LoVo cells. Additionally, MTT also shown depressed DLC-1 gene expression resulted in inhibition of LoVo cell proliferation. Cell migration assay confirmed that knocking down DLC-1 gene expression was followed by promoting in LoVo cell migration. Our observations suggest that DLC-1 gene was associated with LoVo cell proliferation and migration. DLC-1 is a potential suppressor gene in colon cancer LoVo cell line and may play an important role in colon cancer mechanism.
RNA interference DLC-1 gene proliferation cell migration
Peiling Huang Yueling Jin Xiaoqiang Tian Yanfang Shang Peilin Huang
Department of Pathology, College of Medicine, Southeast University, Nanjing 210009, P.R China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）