Enhanced Ginsenoside Production in Ginseng Hairy Roots by Antisense Suppression of Cycloartenol Synthase Gene

摘要：

Antisense suppression of cycloartenol synthase (CAS) gene was employed to improve the ginsenosides level in hair root of Panax ginseng. Transgenic ginseng hair root lines with suppressed CAS expression were obtained by introducing a transgene expressing antisense coding region (0.9kb) of CAS cDNA under the regulation of the 35S CaMV promoter. The resultant transgenic hair root had reduced levels of CAS mRNA and higher levels of ginsenosides. Suppression of CAS also suppressed the growth of ginseng hair root to some extent.

关键词： Antisense suppression cycloartenol synthase ginseng hairy root ginsenosides

作者: Yan-Long Liang Shou-Jing Zhao

作者单位: College of Biological and Agricultural Engineering, Jiling University, Changchun 130022,China

会议类型: 国际会议

会议名称: The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)

会议地点: 苏州

会议语种:英文

在线出版日期: 2007-09-10（万方平台首次上网日期，不代表论文的发表时间）