Expression of hGITR<aa27-165> Gene and Preparation, Detection of its Polyclonal Antibody
Human glucocorticoid-induced tumor necrosis factor receptor (hGITR) is a type I transmembrane protein with homology to TNF receptor superfamily members. Ligation of murine GITR can enhance immunity reactions via several signal pathways. However, so far the certain function of hGITR is not clear. In this study, we constructed pQE30-hGITRaa27-165 prokaryotic expression plasmid and then expressed hGITRaa27-165 fusion protein, subsequently, prepared polyclonal antibody (pAb) against the fusion protein. ELISA, Western blot and FCM were used to identify the titer and specificity of the pAb. The effect of anti-hGITRaa27-165 pAb on the proliferation of human CD4+T cells was investigated by 3H-TdR incorporation test.
GITR prokaryotic expression polyclonal antibody CD4+T cells
Jia Tong Sheng-Jun Wang Jun Chen Chao-Ming Mao Yun-Liang Yang Min Yang Qi-Xiang Shao Hua-Xi Xu
Department of Immunology, School of Medical Technology, Jiangsu University Department of Ophthalmology, the Affiliated Hospital of Jiangsu University, Zhenjiang, China, 212013
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）