Gene Expression Profiles of As2O3-Treated cervical cancer Hela Cells
Arsenic trioxide (As2O3) was found to induce apoptosis in certain types of cancer cells including acute promyelocytic leukemia, and recently in solid tumors.Our previous studies have shown that As2O3 inhibits the growth of human lung adenocarcinoma cell line AGZY-83A and human cervical cancer cell line Hela. To better understand the precise molecular mechanism(s) by which As2O3 exerts its effects on solid tumor cells, we utilized cDNA microarray to interrogate 5,376 known genes to determine the gene expression profiles altered by As2O3 treatment. We found a total of 311 genes that showed significant change after As2O3 treatment from two independent experiments. Among these genes, 239 genes were downregulated and 72 genes were up-regulated with As2O3 treatment.We found that As2O3 regulated the expression of genes that are critically involved in the regulation of cell growth, cell cycle, apoptosis, transcriptional regulation.We conclude that As2O3 affected the expression of a large number of genes that are related to the control of cell survival and physiologic behaviors. The gene expression profiles provide comprehensive molecular mechanism(s) by which As2O3 exerts its pleiotropic effects on cancer cells. As2O3-induced regulation of these genes may be further exploited for devising chemopreventive and/or therapeutic strategies for cervical cancer.
Arsenic trioxide Hela cells Gene expression Microarray
Xue-Feng Gu Jong-Ho Lee Ke-Shen Li Jeong Won Jahng
Dental Research institute, Seoul National University School of Dentistry, Seoul, 110-744, Korea Department of Biomedical Engineering, Harbin Engineering University, Harbin, China, 150001
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）