Identification of Type-Common and Neutralization Epitopes on Hepatitis E Virus Genotype 4 by Using Monoclonal Antibodies
(0) To identify and to characterize neutralizing antigen epitopes of a newly identified Chinese strain of genotype 4 of hepatitis E virus (HEV) and to compare the epitopes with those of HEV strains clustered into other three different genotypes. Recombinant capsid protein p166Chn and monoclonal antibodies (mAbs) against the p166Chn were prepared. Neutralizing activity of the mAbs was tested by an in vitro PCR-based HEV neutralization assay. The neutralizing antigen epitopes in the genotype 4 isolated in China and other strains with different genotypes were analyzed by ELISA and Western blotting methods combined with an additive ELISA assay. Six hybridoma cell lines secreting specific mAbs against p166Chn were obtained.The mAbs could effectively neutralize the infectivity of the homogenous HEV strain to PLC/PRF/5 cells.They strongly reacted with seven different p166 recombinant proteins including p166Chn, p166Bur,p166Mor, p166Pak, p166Mex, p166Us and p166Nz, which were derived from different HEV reference strains of genotype 1, 2, 3 and 4, respectively. Moreover, the additive ELISA assay showed negative reactions of mAbs. The data indicated that all the mAbs recognized a common neutralizing antigen epitope on the p166 proteins. The newly identified HEV genotype 4 possesses the same neutralizing antigen epitope as that derived from HEV strains of genotype 1,2 and 3 worldwide.
Hepatitis E virus neutralization epitope monoclonal antibodies genotype
Hongmei Zhang Xing Dai Xiangnian Shan Jihong Meng
Department of Microbiology and Immunology, Southeast University School of Medicine, Nanjing, 210009, Department of Dermatology of ZhongDa Hospital, Nanjing, 210009 Genetic Research Center of Southeast University, Nanjing210009,Chin Department of Microbiology and Immunology, Southeast University School of Medicine, Nanjing, 210009,
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）