PROLIFERATION AND LOCATION OF HANTAAN VIRUS IN GAMASID MITES AND CHIGGER MITES:A MOLECULAR BIOLOGICAL STUDY
(0) Objective To study the proliferation and location of Hantaan virus (HV) in gamasid mites and chigger mites, and confirm the significance of gamasid mites and chigger mites as vectors of transmission of hemorrhagic fever with renal syndrome (HFRS). Methods Gamasid mites collected from the nests of wild rodents and chigger mites collected from the bodies of wild rodents and thick growth of grass in the field were raised. Two oligonucleotide primers were designed based on the gene fragments of cDNA of HV 76-118 strain to be used in RT-PCR. RNA was extracted from the suspension of the gamasid mites from the nests where no HV had been found in rodents whose lungs lived and from the unfed larvae of chigger mites, being formed groups of 5, 10, 30, or 50 individuals. RT-PCR was conducted to detect the HV-RNA in such suspension. The larvae, nymphs, and imagines of both kinds of mites were collected to make suspension at an interval of 20 days. Vero-E6 cells were inoculated to measure the titer of 50％ tissue culture infective dose (TCID50)/mL of HV. Frozen sections of larvae, nymphs, and imagines of both kinds of mites were made. RT-PCR and in situ hybridization were conducted to detect the distribution of HV-positive particles. Monoclonal antigen technique was used to compare the antigenicity of the HV-RNA from the rodents, mites, and patients from the same epidemic areas. Results HV-RNA was detected in gamasid mites and chigger mites. Except in the larvae of chigger mites 60 days after collection titers of HV were detected and increased gradually in mites at different stages of life cycle. HV-RNA positive particles were detected in the epithelial cells of midgut and ovary with the signal denser and more numerous in nymphs than in larvae. The genotypes of HV from rodents, mites and patients in the same endemic areas were identical HTN type virus. Conclusion HV can be transmitted transstadially and proliferated in mites, gamasid mites and chigger mites play a key role as vectors of transmission for HFRS.
Trombiculid mites Hantaan virus Proliferation Location Vectors of transmission
Yun Zhang Xiao-zhao Deng Guang-Hua Wu Jin Zhu Ke Xu Jing-Jing Yang
The institute of Militalry Medicine, Nanjing Command, Nanjing 210002, China; Department of Epidemio The institute of Militalry Medicine, Nanjing Command, Nanjing 210002, China Department of Epidemiology in Nanjing Medical University, Nanjing 210029.
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）