The Application of nest RT-PCR in Testing the Expression of Tcll and S16 in Single Mouse Oocyte and Peri-implantation Embryo

摘要：

Objectives: To discuss the way to test gene expression in single mouse oocyte and embryos. Methods: The Nest RT-PCR was used to test the Tcll and S16 expression in mouse oocytes and peri-implantation stage embryos. Results: The nest RT-PCR amplification of mouse oocytes, sperms and cumulus cells suggested that both Tcll and S16 expressed in single oocyte, and only S16 expressed in sperm and oocyte. The nest RT-PCR amplification of peri-implantation stage embryos suggested that both Tcll and S16 expressed in pronuclear stage, 2-cell stage, 4-cell stage and 8-cell stage embryos, morulas and blastocyst. Conclusion: The nest RT-PCR can be used to test gene expression in single oocyte and early embryos.

关键词： oocyte cell embryo mouse single cell nest RT-PCR

作者: Haixiang Sun Linjun Cheng Ningyuan Zhang Junxia Wang Bing Wang Yali Hu

作者单位: Reproductive Medicine Center of Drum-tower Hospital affiliated to Medical School of Nanjing University，Nanjing, Jiangsu, Peoples Republic of China

会议类型: 国际会议

会议名称: The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)

会议地点: 苏州

会议语种:英文

在线出版日期: 2007-09-10（万方平台首次上网日期，不代表论文的发表时间）