The Construction and The Biosafety Research of A DNA Vaccine pPCV-HP Against Taenia solium cysticercosis
Our previous study has confirmed that the fragment of HPREαβ1 in HBV genome could obviously improve the gene expression in the post-transcriptional level. In our present study, we constructed a DNA vaccine pPCV-HP, and studied the protection and biosafety of this vaccine. We constructed the new vaccine by inserting the fragment of HPREαβ1 into the downstream of the gene pPCV which is located in the DNA vaccine against cysticercosis. After inoculation to BALB/c mice with pPCV-HP, antibody titers were assayed by ELISA and the mice were performed challenge with the infectious T.solium eggs. Numbers of cysticercus were calculated after three months. Pigs were immunized with the new vaccine using intramuscular injection. Different tissues were separated and the total DNA were extracted from tissues. Then the distribution of the DNA vaccine in different tissues and the possibility of DNA vaccine integration into the host genome were analyzed by PCR. Meanwhile, the environment samples were collected, the transformation and diffusion possibility of CMV promoter gene, kanamycin resistance gene and antigen gene into the environment was analyzed by PCR. Results showed that the antibody titers and the protective rate after immunized with DNA vaccine pPCV-HP were obviously higher than the immunity group of pPCV. The integration of DNA vaccine into host genome and the transformation to environment were not observed in sensitivity of 30 copies. All these revealed that DNA vaccine pPCV-HP has high protective rate and biosafety.
DNA vaccine biosafety genome integration
Wen-Juan Yao Xiao-Zhao Deng Zhen-Yu Diao Hui Zhong Zong-An Zhou Yu Liu
College of Biological Science and Technology, China Pharmaceutical University, Nanjing 210009, China The Institute of Militalry Medicine, Nanjing Command, Nanjing 210002, China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）