The Effect of Induced Anti-FSH Autoantibody on Rat Testis
To research the apoptotic mechanism of the spermatogenic cells in the model rat induced by anti-FSH autoantiboby. Method: The serum FSH, LH and inhibin B levels were measured by enzyme-linked immunosorbent assay (ELISA). The apoptosis of spermatogenic cells were detected using the in situ end labeling method (TUNEL). We also determined the intratesticular Bax mRNA level in model rat by Fluorescent Quantitative PCR. Result: The serum FSH level in model rat immunized with polypeptide for 77 d and 91 d was significantly lower than that in controls (p< 0.05). However, the serum FSH level increased to normal in the model rat immunized with polypeptide for 105 d. Compared with the controls, the serum inhibin B level was significantly declined (p< 0.05). The serum LH level had no difference between control and model rat. The intratesticular Bax mRNA level in model rat immunized with polypeptide for 77 d, 91 d and 105 d was 2.15, 3.77 and 1.89 fold of controls respectively. The result of TUNEL detection showed that the apoptotic index of spermatogenic cells in model rat was significantly higher than that in controls. Conclusion: The decreased serum FSH level caused by anti-FSH autoantibody could induced the apoptosis of spermatogenic cells by the signal pathway involved in Bax.Follicle-stimulating hormone (FSH) was required for initiation of spermatogenesis during prepubertal stage and it played an important role in maintenance of adult spermatogenesis 1, 2. It had been known that in male FSH receptor was specifically expressed only in the Sertoli cell, the unique soma contacted with germ cells. Under the stimulation of FSH, Sertoli cell could secrete active materials regulating the growth and development of spermatogenic cells 1, 3. Blocking the activity of FSH using passive immunization resulted in the impairment of spermatogenesis 4, 5. Our previous study 6 indicated that the incidence of the anti-FSH autoantibody in the patients with oligospermia and azoospermia was significantly higher than that in fertility men. We presumed that the anti-FSH autoantibody might affect the spermatogenesis of the male testis. To further research the effect of anti-FSH autoantibody on male spermatogenesis, in our current study we established the rat model of anti-FSH autoantibody (unpublished paper). Briefly, A specific polypeptide representing amnio acid residues 19-36 of the rat FSH ?-subunit was synthesized and coupled to KLH (keyhole limpet hemocyanin). Rats were immunized using polypeptide-KLH (model group) and KLH (control group) respectively. Further immunization was performed every 2 weeks. After immunized for 63 d, the titer of serum anti-polypeptide antibody was 1: 400 detected by ELISA and the optical density of the polypeptide antiserum significantly reduced after being absorbed with purified rat FSH. Compared with control group, we had found that the number of spermatogenic cells in seminiferous tubule and the sperm counts in tubular lumina decreased (unpublished paper). Based on our previous experimental results, the current research was to explore the apoptotic mechanism of the spermatogenic cells in the model rat induced by anti-FSH autoantiboby.
Yao Bing Liang Wei Cui YingXia Ge Yifeng
Institute of Clinical Laboratory Medicine, Nanjing Jinling Hospital, Clinical School of Medical Coll Department of Chinese Traditional Medicine, No.454 Hospital, Nanjing 210002, People’s Republic of Ch
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）