The long term cultured of Human B cells from PBMCs stimulated via Soluble CD40L for the enhancement of antigen presentation in vitro
We aimed to set up a method for long-term culture for B cells and to investigate whether B cells may be activated by soluble CD40 ligand (sCD40L) and loaded with virus peptide. B cells could be cultured in the presence of sCD40L and recombinant human interleukin-4 up to 50 days. The B cells in the S phase increased from 0％ to 8.34％. The numbers of CD80, CD86, MHC classes I and II molecules on the sCD40L-activated B cells were significantly increased. Fluorescence microscopy showed that the activated B cells had strong fluorescence after peptide pulsing. Cytometry displayed that more than 98％ scd40L-activated B cells were load by the hepatitis B core peptide. The results suggested the human B cells activated by sCD40L strongly up-regulated surface molecules on the B cells and had the ability to present antigen. Therefore, B cells may function as antigen presenting cells.
B cells Soluble CD40 ligand Antigen presentation
Chao Wu Yong Liu Qi Zhao Guang-Mei Chen Jun-Hao Chen Xiao-min Yan
Departments of Infectious Diseases1 and Laboratory Medicine Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）