Focal Adhesion Kinase Expression: A Potential Tumor Therapy Target
The focal adhesion kinase (FAK) is a nonreceptor protein tyrosine kinase implicated in cell cycle progression and migration. Over-expression of FAK in a variety of tumors suggested that FAK is a promising target for therapeutic intervention. In this study, we took advantage of a modified polyethylenimine (M-PEI) with high transfection efficiency for tumor cells and tissues, and targeted FAK function through two approaches both in vitro and in vivo. The results demonstrated that both the plasmid encoded FAK siRNA and over expressing of FAK-related non-kinase (FRNK, FAK dominant negative) dramatically inhibited in vitro B16-F10 cells proliferation and invasion. For the purpose of evaluating the therapeutic potential of PEI-complexed plasmids targeting FAK function, two transplantable mouse tumor models of primary and metastatic melanoma were adopted in this study, the results of which also revealed that intratumoral delivery of PEI-complexed plasmids targeting FAK significantly suppressed the primary tumor growth as well as the metastasis of B16-F10 cells into lung and lymph nodes. Both approaches protected the tumor-bearing mice with a prolonged survival. Taken together, these results indicated that intratumoral delivery of plasmid DNA targeting FAK function represents a promising avenue for melanoma therapy by using M-PEI as a gene carrier.
FAK FRNK RNA interference Polyethylenimine
Shufeng Li Wei Dong Yiwei Zong Wu Yin Guanghui Jin Qingang Hu Xiaofeng Huang Wenhui Jiang Zichun Hua
The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science, Nanjing Universit The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science, Nanjing Universit The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science, Nanjing Universit The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science, Nanjing Universit
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）