The Study on Apoptosis and Immunohistochemical Localization of Annexin 5 in Rat Cryptorchid Testis
Aim: To investigate the expression of annexin 5, the distribution of annexin 5 and 3b-HSD and apoptosis of germ cells in cryptorchid testis. Methods: After cryptorchid surgery, abdominal and scrotal testes were removed at 96h, 1 w, 2 w and 4 w respectively. Immunohistochemistry, Western-blot and TUNEL were used. Results : Results showed that the annexin 5 was increased significantly in abdominal testis at 1 w (p<0.05) and at 4 w (p<0.01). Annexin 5 was appeared mainly in intra seminiferous tubules at 1 week and was aggregated with stronger intensity at 2 week in cryptorchid testis. The sperm was disappeared and the number of spermatocyte was reduced from 1 w of surgery. Annexin 5 in all scrotal testis was localized mainly in interstitial tissue and Setoli cells. 3b-HSD in abdominal testis was diminished at 1 d and disappeared at 1 w of cryptorchidism. Apoptosis in abdominal testes was found at 1d and increased at 1w. In scrotal testis, no apoptotic cell was found. Conclusions: As a conclusion, the variation of expression and distribution of annexin 5 in cryptorchid testis, as well as the decrease of 3b-HSD and apoptosis in abdominal testis implied on that annexin 5 may play a certain physiologic role in abdominal testis after experimental cryptorchid surgery.
cryptorchid annexin 5 3b-HSD
Zhu Huang Chao Jiang Yao B
Institute of Clinical Laboratory Medicine, Nanjing Jinling Hospital, Clinical School of Medical College, Nanjing University, Nanjing 210002, People’s Republic of China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）