Relationship between genetic susceptibility of non-small cell lung cancer and TGF-βRI gene
investigate whether the transforming growth factor-beta receptor type I(TGF-βRI) gene is associated with genetic predisposition of primary non-small cell lung cancer (NSCLC). The entire coding region of TGF-βRI and flanking intron sequences from 53 NSCLC tissues were examined for alterations using SSCP and direct sequencing. No somatic point mutations other than two silent mutations and a polymorphism were found in the TGF-βRI gene. The two silent mutations located at codon 544 (AAT to AAC) and codon 406 (TTA to CTA), respectively, and the polymorphism was at 24 th base of intron7 (G to A). Interestingly, we found that the subjects with homozygous genotype A/A displayed more than threefold increased risk of developing NSCLC than the common wild genotype G/G. As the first report, this study showed that TGF-βRI gene is not a frequent site of spontaneous mutational inactivation while the detected polymorphism could be a susceptibility allele that predisposes to carcinogenesis of NSCLC.
TGF-βRI NSCLC PCR-SSCP Mutation Polymorphism
ZHENG Shi-Yin ZHANG Hong-Tao ZHAO Ju XU Ling-Yan
Department of Cardio-Thoracic Surgery, The First Affiliated Hospital of Suzhou University,Suzhou 215 Laboratory of Medical Genetics, School of Life Sciences, Suzhou University, Suzhou 215007, China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）