Effect of Bisphenol A on steroid production in human granulosa cells, a study in vitro
To study in vitro the effects of environmental estrogen disruptor, bisphenol A (BPA), on ovary function with the model of the cultured human granulosa cells. Materials and Methods Granulosa cells from healthy women were collected and cultured. BPA, final concentration 10-7 to 10-4M, was added to the cell-culture medium to treat cells for 48 hours. The levels of estrogen and progesterone were measured by DELFIA. The expression levels of P450scc and P450arom were measured by RT-PCR. Results The granulosa cells expressed high levels of P450scc and P450arom and produce high levels of estrogen and progesterone. After BPA treatment, expression of P450arom was reduced significantly at 10-5 to 10-4M of BPA, while expression of P450scc definitely at 10-6 to 10-5M of BPA. The levels of estrogen and progesterone were not affected significantly by BPA at the present concentrations and within 48 hours. Conclusion: BPA could affect steroid hormone synthesis and transformation in granulosa cells, such as the expression of P450scc and P450arom, so it affects ovarian function.
human granulosa cells bisphenol A ovary steroid hormone
Nancy Jiang Yu-Gui Cui Jia-Yin Liu
Center of Clinical Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical Universit Center of Clinical Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical Universit
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）