EXPRESSION OF HEART DEVELOPMENT-ASSOCIATED GENES IN MESENCHYMAL STEM CELLS CO-CULTURED WITH CARDIOMYOCYTES
Recent studies have demonstrated that bone marrow mesenchymal stem cells (MSCs) have the potential to transdifferentiate into cardiomyocytes (CMs) in an appropriate condition both in vivo and in vitro. However, there exists a controversy among a variety of studies about whether MSCs can differentiate into CMs without physical contacts with CMs, and the molecular signals that underlie this process are not fully understood. In the present study, MSCs were cocultured with CMs in a dual chamber dish separated by a semipermeable membrane for 2 weeks. During this non-direct contact coculture procedure, cardiomyogenic differentiation and relative genes expression in MSCs were evaluated. After coculture with CMs, MSCs showed a stick-like or elliptical morphology. Immunofluorescent stain results revealed that α-actin and cardiac troponin T(cTnT) positive cells were found in MSCs at 5 days after coculture with CMs. Results from RT-PCR demonstrated the expression level of TGF-β, Nkx-2.5, GATA-4 and MEF-2C genes began to increase at 1 day and reached the peak at 7 days after coculture. In conclusion, non-direct contact with CMs (1:5) is conducive for MSCs to differentiate into CMs in vitro, and TGF-β, Nkx-2.5, GATA-4 and MEF-2C genes may play a crucial role during the transdifferentiation.
bone marrow mesenchymal stem cells non - direct contact differentiation genes cardiomyocytes
Junyi Zeng Yang Wang Yunfeng Wei An Xie Yuanlei Lou Mei Zhang
Department of Cardiology,the First Affiliated Hospital of Nanchang University, NanChang 330006,China Institute of Urological Surgery, the First Affiliated Hospital of Nanchang University, NanChang 3300
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）