Identification of SNP on the ITS and 5.8s Gene of Panax Species by ALM-ASA
An accurate SNP indentification method of Panax species was established by searching SNP sites in Genebank. Based on the SNP on the ITS and 5.8s gene, specific primers were designed for Panax species. Using Adapter-ligation Mediated Allele-specific Amplification(ALM-ASA)method to detect the type of SNP. After optimizing the PCR reaction system, the sizes of amplicon from P. quinquefolius, P. japonicus, P. notoginseng and P. ginseng template are 111bp, 145bp, 245bp and 294bp respectively by using the allele-specific primers. The results showed that the method was specific, reproducible and could be carried out in a single tube. By the ALM-ASA technique，n+1 primers(n specific primers and a universal primer)can be used for an n-plex PCR amplification；the specificity of PCR is thus enhanced significantly. The ALM-ASA method can be used for typing multiple SNPs simultaneously. It is quite feasible to use the SNP typing of ITS and 5.8s gene as a criterion for the identification of Panax species. The method also supplies a reference for the evluation of quality.
Panax species Single nucleotide polymorphism(SNP) ITS and 5.8s gene Adapter ligation-mediated allele-specific amplification(ALM-ASA)
Song Qinxin Zhou Guohua
Department of Pharmaceutical Analysis，China Pharmaceutical University，Nanjing 210009 Department of Pharmaceutical Analysis，China Pharmaceutical University，Nanjing 210009; Huadong Resea
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）