One-dimensional Microfluidic Beads Array for Nucleic Acids Detection
(0) A one-dimensional addressable beads array for nucleic acids detection is presented in this paper. Molecular beacons (MBs) modified porous silica micro-beads were deposited along a micro-channel in sequence. This MB-functional beads array cooperating with microfluidic control allows multiple nucleic acid targets detection simultaneously with only once samples injection,a rapid dynamic hybridization and no need of samples labeling.As a demonstration, the synthesized DNA targets according to the same sequence with a section of human gene p53 mRNA was investigated with a limit of detection as few as 0.5 nM. We have further examined the asymmetry PCR (a-PCR) products of human gene p53, c-myc and cyclin-d1 to detect the mRNA expression changes before and after anticancer drug 5-flouorouracil (5-FU) treatment. Such a platform used for nucleic acids detection has shown its advantages of handling simply, little samples consumption, low potential of contaminations, and low cost. Also, it have a potentially application to pursue highthroughput nuclei acids detection in a single channel.
microfluidic beads array molecular beacon nucleic acids
Xinbing Zuo Xiaohai Yang Kemin Wang Jianhui Wen He Zhang
State Key Lab of Chemo/Biosensing & Chemometrics, Biomedical Engineering Center, College of Chemistry & Chemical Engineering, Key Lab for Bio-Nanotechnology & Molecular Engineering of Hunan Province, Hunan University Changsha 410082, China
国际会议
武汉
英文
140-142
2007-07-06（万方平台首次上网日期，不代表论文的发表时间）