Expression of a Novel Chitinase Gene from Trichoderma harzianum in Saccharomyces cerevisiae
Full-length cDNA encoding endochitinase Chit37 was obtained successfully by construction of T. karzianum mycelium eDNA library and sequencing and bioinformatic analysis of the random selection clones. The results indicated that the open reading frame of Chit37 was 1, 032 bp encoding a protein of 343amino acids and the deduced molecular weight was 36.6 kDa.The gene was amplified by PCR and then integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/Chit37 which was used to transform S. cerevisiae H158. Chitinase expressed by pYES2/Chit37 was induced by galactose (maximal activity 0.75units ml-1). The enzyme activity reached the peak expression after cultured for 96 h. The optimal temperature of Chit37 is 40℃ and the optimal pH is 5.0.
T. harzianum Chitinase Expression
Huang Caihong Yang Qian Song Jinzhu Song Yingqi
School of Science Harbin Institute of Technology Harbin, China
国际会议
武汉
英文
287-289
2007-07-06（万方平台首次上网日期，不代表论文的发表时间）