A New Optimized Spectrophotometric Assay for the Measurement of Pyruvate Dehydrogenases Activity
Pyruvate dehydrogenase is a key enzyme which plays a pivotal role in energy metabolism. In most of studies on pyruvate dehydrogenase, it is neccessary to measure the enzymatic activity of them. It was found in our previous study that existing assays for pyruvate dehydrogenase activity may not reflect exactly enzymatic activity on account of some negative interferences. We therefore developed a new spectrophotometric assay which is applicable for the measurement of pyruvate dehydrogenase activity in both purified pyruvate dehydrogenase and pyruvate dehydrogenase in Pruvate dehydrogenase complex isolated mitochondria. Our findings suggest that the assay procedure has higher sensitivity and practicability than that of previous assay procedures.
pyruvate dehydrogenase enzymatic activity measurement of enzymatic activity
He Yahui Li Yan He Hongwu Yuan Junlin
Laboratory of Environmental Science College of Life Science, Huazhong Normal University Wuhan, China
国际会议
武汉
英文
424-427
2007-07-06（万方平台首次上网日期，不代表论文的发表时间）