Influence of Di-(2-ethylhexyl) Phthalate on Mice Digestive System
This experiment was about the effect of celiac injected di-ethylhexyl phthalate (DEHP) on DNA damage in mice stomach cells by using single cell gel electrophoresis (SCGE). In the present study, mice stomach cells were isolated as experimental materials and comet assay was applied to determine the DNA damage induced by celiac injection of DEHP (exposure concentrations were 62.5mg/kg, 125mg/kg, 250mg/kg, 375mg/kg,500 mg/kg) in vivo for two weeks. The results revealed that DEHP could induce DNA damage of mice gastrointestinal tract and DNA damage was elevated along with the rising of concentration of DEHP. When the exposure concentration was 125mg/kg, DEHP could induce DNA strand breaks significantly (p＜0.05), and when the exposure concentration was 250mg/kg and 375mg/kg, DEHP could induce DNA strand breaks significant (p＜0.01), but when the exposure concentration was 500mg/kg, DEHP could induce DNA and protein crosslinking.The experiment suggested that DEHP can induce the breaks and crosslink of DNA in the cells of gastrointestinal tract.
di-(2-ethylhexyl)phthalate (DEHP) comet assay stomach cells
Yang Lu Li Xuebin Guo Jianjun Ding Shumao
Laboratory of Environmental Science College of Life Science, Huazhong Normal University Wuhan, China
国际会议
武汉
英文
475-477
2007-07-06（万方平台首次上网日期，不代表论文的发表时间）