A Study on DNA Breaks and DNA-protein Crosslinks Induced by Formaldehyde in two Types of Mice Cells
Objective: Previous studies have confirmed that formaldehyde (FA) is genotoxic and mutagenic to mammalian cells. FA can induce a broad spectrum of genetic effects. This study is undertaken to investigate the direct effect of formaldehyde on two types of mice cells. Methods: The single cell gel electrophoresis (SCGE) and KCl-SDS assay were used to detect the effect of FA in mice lung cells and mast cell line (P815) in vitro. Results: The effects of formaldehyde in the two types of cells were similar. The results showed that liquid formaldehyde induced DNA breakage at 5 μM, 25 μM (P<0.01, compared with control group) and the DNA-protein crosslinks were found at high concentrations (125 μM P<0.05; 625 μM P<0.01) Conclusions: The results showed that formaldehyde caused DNA breaks and crosslinks for different types of mice cells.
formaldehyde genotoxicity DNA-protein crosslinks DNA single strand breaks
Yang Guangtao Wu Kai Lou Xiaohua Wang Liming Ding Shumao Yuan Junlin Yang Xu
Laboratory of Environmental Science, College of Life Science, Huazhong Normal University, Wuhan 430079
国际会议
北京
英文
2007-09-07（万方平台首次上网日期，不代表论文的发表时间）