Heritability, combining ability and heterosis in glucosinolate content in seed of winter rape (Brassica napus L.) estimated with diallel crosing between double haploid lines
Six DH lines with very different glucosinolate content were crossed in complete diallel design. Obtained hybrids of F1 and F2 generations and parental lines were grown in field trial in complete random block design in replications. Analyses of glucosinolate content and composition were made using gas chromatography of sillyl derivatives of desulphoglucosinolates. Calculation of GCA and SCA were performed according to Griffings method. Analysis of variance showed that the GCA effects for the investigated glucosinolate were statistcally very significant. Significant effects of SCA were found for gluconapin and progoitin.SCA for 4-hydroxybrassicin was not significant. Heterosis effects were calculated for pedigrees of individual parents and for hybrids as compared with parent means. Highly significant heterosis effects in F1 generation lost its significance in F2 generation.Variance analysis according to Hayman showed very significant additive effects of genes for the examined glucosinolate.Different methods for estimation of heritabilities of glucosinolate contents in seeds were compared. The expected heritabilities were calculated according to Mather in a narrow and wide sense. Realized heritabilities between F1 and F2 generations were investigated by calculations of regression coefficient, correlation coefficient and determination coefficient. The best heritability estimator should be determination coefficient because both generations were grown in different conditions during two following years. Good agreement was found for heritabilities in narrow sense (h2=0.914) and determination coefficient (r2=0.908) for progoitrin content and slightly worse for gluconapin (h2=0.868, r2=0.913). Heritability in a narrow sense according to Mather for 4-hydroxybrassicin was not confirmed by determination coefficient between F1 and F2 generations.
heritability GCA SCA heterosis variability alkenyl glukosinolate indol glucosinolate DH lines
Teresa Pietka Krystyna Krotka Jan Krzymanski Teresa Cegielska-Taras
Plant Breeding and Acclimatization Institute, Department of Genetic and Breeding of Oilseed Crops, Strzeszynska 36, 60-479 Poznan, Poland
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
73-76
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）