Breeding of yellow seeded Brassica napus L. var. oleifera via wide crosses in in vivo and in vitro conditions
The crossability in inte rspecific crosses of male sterile (MS) and fertile B. napus var. oleifera (2n =AACC =38) with four yellow and brown seeded Brassica species i.e.B. campestris ssp. trilocularis (yellow seeded turnip rape, 2n =AA =20), B.campestris ssp. pekinensis (brown seeded pekinensis cabbage, 2n =AA =20), B. hirta (white mustard, 2n =SS =24) and B.carinata (Ethiopian mustard, 2n =BBCC =34) in in vivo and in vitro conditions was investigated. Crossing of over mentioned species was done in the greenhouse and in vitro culture laboratory of the Department of Genetics and Plant Breeding of Agricultural University in Pozna(n), Poland. The crossablity between used species was evaluated based on the observation of pollen grains germination, pollen tube growth and seed set in in vivo conditions and embryo set in the ovules after in vitro pollination. Hybrid F1 seeds and hybrid F1 plants regenerated in embryo culture were obtained from four of five cross combinations. Crossing of B. napus with white mustard was quite ineffective. The hybrid F1 plants obtained after crosses of fertile B. napus with other Brassica species were male fertile, while those emerged after pollination of MS mother line were male sterile.However, they set the seeds after back crossing with paternal initial form. The observation of microsporogenesis in F1 hybrid plants showed that they formed normal tapetum and archespore tissue but there were disturbances in chromosome conjugation and their distribution into descendant nuclei. Effectivenes of crossing was different in particular cross combinations both in in vivo and in vitro conditions.
Brassica seed colour interspecific crosses embryo culture
Andrzej Wojciechowski Blazej Springer Jan Olejniczak
Department of Genetics and Plant Breeding, Agricultural University in Pozna(n), Poland Institute of Plant Genetics, Polish Academy ofSciences, Pozna(n), Poland
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
191-193
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）