Genomic in Situ hybridization in intergeneric hybrids between Raphanus sativus and Brassica oleracea
Genomic in situ hybridization (GISH) was applied to study the meiosis of F1 plants from intergeneric hybrids between radish (Raphanus sativus L., 2n=18, RR) and cabbage(Brassica oleracea L., 2n=18, CC). The result showed that its somatic cells had the expected chromosomes, RC, 2n=18; but that the pollen mother cells (PMCs) were different. There were three main kinds of PMCs. The first one was RC(2n=18), and the mean chromosome pairing pattern was: 14.871+1.20Ⅱ+0.04Ⅲ+0.06Ⅳat Diakinesis.GISH indicated that most bivalents resulted from chromosome pairing between radish and cabbage, and that the nine chromosomes of R-genome were separated mostly in the ratio 5/4 and 6/3 at Anaphasel, so the chromosome number and components in gametes were not in equilibrium and the gametes were sterile. The second was RRCC (2n=36) with normal chromosome pairing and separation, producing unreduced gametes. And the third was hypoploid of RRCC (2n＜36=30～35).GISH showed that some radish chromosomes were lost in those PMCs, and that its gametes had nine cabbage chromosomes and partial radish chromosomes. Cytological characterization of BC1 hybrids suggested that the unreduced gametes both RC euploid and its hypoploid could be transmitted to progenies by female parents.
Raphanus sativus Brassica oleracea Intergeneric hybrids Cytogenetics GISH
CHENG Yugui WU Jiangsheng ZHANG Minghai FANG Huaming
National Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University, Wuhan 43007 National Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University, Wuhan 43007 Yichang Institute of Agricultural Science of Hubei Province, Yichang Hubei, 443004, China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
296-301
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）