Effect of 12C heavy ion beam irradiation on rapeseed (Brassica napus)
After treating of 30Gy, 50Gy and 80Gy 12C heavy ion beam irradiation on rapeseed, the effects of botanical characters,quality characters, chromosome behavior and DNA molecular polymorphism were studied. The results are as follows: Treatments of 50Gy and 80Gy 12C heavy ion beam irradiation can make the growth duration earlier and change the botanical characters of rapeseed. These botanical characters include larger leaves of the plant, luxuriant growth, improved agronomy characters. Some plants have variations such as tumor-like root, dwarf stem, light green spoon leave, multi-pistil flower, multi-silique and yellow see& Treatment of 80Gy 12C heavy ion beam irradiation increases oil content to different degree. The oleic acid contents of some plants are even higher than 70％. Both root top chromosome aberration and pollen mother cell chromosome aberration occur under 30Gy, 50Gy and 80Gy 12C heavy ion beam irradiation on rapeseed. The aberration type includes micronucleus, mininucleus,abnormal tetraspore, bridge, lagging chromosome and fragment, most of which are micronucleus. Treatments of 50Gy and 80Gy 12C heavy ion beam irradiation have effects on molecular biology of rapeseed. By using 40 random primers to amplify the rapeseed after the above treatment, 43 DNA fragments are amplified. It shows polymorphism to some degree in the rapeseed plants after different dosage treatments.
12C heavy ion beam irradiation rapeseed
GUAN Mei LI Xun
Hunan Agricultural University, Changsha, Hunan 410128, China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
306-312
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）