Studies on rapeseed germplasm enhancement by use of cruciferous weed Rorippa indica
Cruciferous weed Rorippa indica has trait of excellent tolerance to soil wateriness and has trait with very short beak or without silique beak. To transfer traits of R.indica to B. napus, we used protoplast fusion and intergeneric hybridization technique for rapeseed germplasm enhancement. Using PEG-DMSO protoplast fusion approach and culturing with B5 liquid medium,somatic fusion hybrids between B. napus and R.indica were obtained. The somatic hybrids were morphologically intermediate types between B. napus and R. indica, could seed. Three-way intergeneric hybridizations, (B. napus×Orychopragmus violaceus)F1×R.indica, were successfully carried out by appropriate embryo rescue approach. Plants morphologically similar to that of R.indica, B. napus, O. violaceus, B. campestris, B. juncea, were observed in F2 generation of the three-way cross, showing crazy segregations. In the population of 890 F2 individuals, 20.9％ was with short-beak less than 5mm. Morphological and cytogenetic identification showed that plants obtained by both ways were new rapeseed germplasm derived from R.indica.
Rorippa indica Brassica napus protoplast fusion intergeneric hybridization
GUAN Rongzhan JIANG Shuhui XIN Ruying ZHANG Hongsheng
National Key Lab of crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R.China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
329-332
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）