Cytology of multisomic addition line NJ04-8089 in Brassica napus L.
A B genome chromosome multi-somic addition line NJ04-8089 in Brassica napus (AACC 2n=38)was identified from offsprings derived from BC1F1of Brassica napus/Brassica carinata (BBCC 2n=34)//B. napus. Results from cytological observation showed that somatic chromosome number was 2n=45 observed from either root-tip or baby ovary cells of NJ048089. 7 univalent scattered around the equator at Metaphase Ⅰ(MI) of pollen mother cells (PMCs) meiosis. A chromosome configuration of 19Ⅱ + 7Ⅰ formed at MI. Some lagged chromosomes formed at Anaphase Ⅰ (AI). The 19 divalent observed could be the result of the homologous pairing of AACC chromosomes and the 7 additional chromosomes were the chromosomes of B genome coming from B. carinata according to known relationships between chromosome of A-A, C-C, A-C, B-A and B-C genome. It indicates that the 7 chromosomes likely that they originated from B genome of Brassica carinata.An unequal chromosome number with a wide range of distribution from 2n=38 to 2n=51 found in root-tip cells of seeds harvested from NJ04-8089 plant after bagging which resulted in an unequal chromosome number in each of the gametes produced from it. Out of the total root-tip observed there was 17.28％, 13.58％ and 20.99％ having achromosome number of 2n=43, 2n=44 and 2n=45 respectively. It indicated that the 7 univalent was randomly assorted to each of the polar at AI but some of them acted as a group staying in one side of the cell in stead of random assorting.
Brassica napus Brassica carinata Multisomie Additional line Cytology
YUAN Shifeng QI Cunkou
Key Laberatory of Plant Germplasm and Breeding Ministry of Agriculture, Jiangsu, Nanjing 210095 Institute of Industrial Crops of Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
364-367
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）