Genetic study of very high glucosinolate content in Ethiopian mustard seeds
The Ethiopian mustard (Brassica carinata A. Braun) line N2-6215, with very high levels of glucosinolates in the seeds (170 μmol g-1 seed), was developed from the line C-101 (125 μmol g-1) following chemical mutagenesis. The objective of this research was to study the inheritance of very high glucosinolate content in seeds ofN2-6215. Plants ofN2-6215 were reciprocally crossed with plants of two lines with standard (C-101,127 μmol g-1) and slightly reduced (S2-1241, 115 μmol g-1) glucosinolate content.The F1, F2, and BC1F1 plant generations were evaluated under the same environment and seeds from individual plants were analysed for total glucosinolate content by near-infrared reflectance spectroscopy. The very high glucosinolate content in N2-6215 seeds was maternally inherited. No cytoplasmic effects were detected. The trait was found to be oligogenic and detemined by at least 2-3 genes. The estimates of heritability were 0.31 in the cross with S2-1241 and 0.35 in the cross with C-101. The total glucosinolate content in leaves of the parents, F1 and F2 plants from the cross C- 101 ×N2-6215 was measurred in order to determine whether the increased glucosinolate levels in the seeds were also expressed at the leaf level. Total glucosinolate content in leaves ofthe N2-6215 parent was 59 μmol g-1, compared to 52 μmol g-1 in leaves of C-101 plants. A moderate but significant correlation (r=-034) between seed and leaf glucosinolate content was observed in F2 plants, suggesting that the trait is not seed specific.
Ethiopian mustard Brassica carinata seed glucosinolate content genetic study leaf glucosinolate content
Angustias Márquez-Lema José M.Fernández-Martinez Bego(n)a Pérez-Vich Leonardo Velasco
Institute for Sustainable Agriculture (CSIC).Alameda del Obispo s/n.E-14004 Córdoba, Spain
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
393-396
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）