Brassica napus plant regeneration and transformation via agrobacterium-mediated-transformation
Gene transformation Technology is a powerful tools for transformation of desirable genes to organisms that use for biology scientists. Transformation of genes which can create a new triat, development of quality or quantity triats had been important for scientist. Rapeseed (Brassica napus) is one of the most important oil seed crop in with usage industrial and edible. Genetic manipulation of rapeseed relies on the suitable tissue culture and efficient method for the plant regeneration and need a good tramsformation system. The results showed that cotyledons were able to multiple shoot formation with high regeneration frequency than hypocotyl in B. napus. In this study two Agrobacterium tumefaciens strains, LBA4404 and C58 (pGV3101), were used for gene transformaion. Cotyledon explants excised from 5 days old seedling in sterile condition and placed onto MS medium containing 4.5 mg/l BAP, 3％ sucrose and 0.8％ agar pH 5.8 for 1 days. The cotyledons were inoculated with suspension of Agrobacterium for 10 seconds and immediately transferred to the same madium for 2 days in dark and 25 ℃ (co-cultivation). Explants were transferred to shoot induction medium with 15 mg/l kanamycine and subcultured each 10 days on the same medium. Green shoots were transferred to elongation medium and after 2-3 weeks green shoots transferred to root induction medium, containing 2 mg/l IBA. Molecular analysis, PCR, and histochemical GUS assay were carried out on the plants. Results showed that 1 day cotyledon preculture was useful for shoot induction and LBA4404 strain was better than C58 (pGV3101) strain for gene transformation.
Brassica napus Tissue culture gene transformation reporter gene Agrobacterium
Alireza Zebarjadi Mokhtar Jalali Javaran Ali Hatef Salmanian Ghasem Karimzadeh
Departement of Plant Breeding and Agromoni, Faculty of Agriculture, Razi University, Kermanshah-Iran Departement of Plant Breeding Faculty of Agriculture, Tarbiat Modares University, Tehran-Iran National Institutefor Genetic Engineering and Biotechnology
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
481-483
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）