THE LATEST IN SEED COAT FASHION: seed colour (proanthocyanidin)and trichome mutations in a new population of activation-tagged Arabidopsis lines
Flavonoids comprise a diverse group of phenolic compounds, which serve a variety of ecological and physiological functions in plants. These include attraction of pollinators, defense against predators and pathogens, and protection against UV damage. Flavonoid polymers (proanthocyanidins, also known as condensed tannins) are advantageous for controlling ruminant digestion and insect foraging. They also have wide-ranging benefits in human health. In the oilseed and vegetable Brassicas, they are present inbrown and black seed coats as a part of the anti-nutritional phenolic fibre fraction and their reduction is the focus of major breeding programmes around the world. Several collections of chemically-induced and T-DNA-induced mutants in the Arabidopsis flavonoid pathway have been screened worldwide to find transparent testa (tt) and tannin-deficient seed (tds) mutants in the proanthocyanidin pathway. These studies have led to a detailed understanding of the enzymology and metabolic regulation of much of the flavonoid pathway. Seventeen flavonoid genes have been characterized over the past decade in Arabidopsis,including six regulatory genes, a vacuole transporter, and eleven structural genes, but genes defining the last steps in polymer formation are unknown. Recently, a new population of activation-tagged Arabidopsis lines (～50,000) and T-DNA -tagged lines (～20,000) were developed in Saskatoon, Canada. Within this population, we have discovered additional seed coat colour alleles,interacting trichome alleles, and several novel proanthocyanidin-free mutants. The lines are being used to re-define the pathway and as a basis for isolating novel genes.
Arabidopsis proanthocyanidin transparent testa tannin-deficient seed mutants
Margaret Gruber Dejun Cui Limin Wu Bruce Coulman Isobel Parkin
Agriculture and Agri-Food Canada 107 Science Cresc., Saskatoon, Saskatchewan, S7N0X2, Canada Agriculture and Agri-Food Canada 107 Science Cresc., Saskatoon, Saskatchewan, S7N0X2, Canada;Dept.of Dept.of Plant Science, University of Saskatchewan.51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
506-509
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）