Improvement of erucic acid level in Brassica napus L. Yudal
The improvement of erucic acid content in high erucic acid rape (HEAR) varieties is one of the important research parts, as increasing of their value as an inhustrial lubricant. Yudal is possible to be used to high eucic acid rapeseed variety in Korea. KCS (3-ketoacyl-CoA synthase) had been proposed as a candidate gene for explaining erucic acid level in rapeseed. To make the Yudal having the erucic acid level more than usually, we have found many fatty acid elongases with database search in Brssica napus, and then divided into two groups by DNA sequence homology. One of these groups were contained the FAE genes of B. napus HEAR Hero and B. olearcea high erucic acid ME103 variety as well as the partial FAE1-1 and 1-2 genes of Yudal. On basis of their DNA sequence, FAE1 cDNA about 1.5kb (YFAE1) has been able to be obtained from Yudal leaves by PCR. This gene have the 2 transmembrane regions predicted by the TMHMM2 program, and is expressed in all organ by RT-PCR, especially high in silique before harvest, and in the petal and calyx among flower organs. Green fluorescent protein (GFP) constructs under Arabidopsis SEN1, or BCB promoter was transformed transiently to rapeseed protoplast to choose the promoter working in rapeseed well besides 35S promoter. These SEN1 and BCB promoters were showed the similar GFP level to 35S promoter. So, we are transforming Yudal with the YFAE1 over-expressing construct under BCB promoter to make the improvement of erucic acid level.
Brassica napus Yudal erucic acid FAE1 overexpression transformation
Yoon Hi, Choy Yu Jeong, Jeong Min Jung, Kim Hyun Mi, Park June Seung, Lee Young Suk, Jang In Sun, Han Moon Sun, Yoon Young Ho, Yoon
Biological department, Natural Science College, Ewha Womans University, Seoul, Korea Mokpo Experiment Station, NICS, RDA, Muan, JeonNam, Korea KCI Limited corp., Siheung-Si, Gyeonggi-Do, Korea
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
530-533
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）