The establishment of high frequency somatic embryogenesis and plant regeneration system of Eruca sativa Mill
A protocol was developed for high frequency somatic embryogenesis and plant regeneration of E. sativa Cotyledon explants grown on MS medium supplemented with 1.0mg/L 2,4-D formed embryogenic callus after 4 weeks culture. Secondary somatic embryos were also produced from that on MS medium containing 0.2mg/L2, 4-D. Somatic embryos differentiated into mature embryos on N6 medium. Mature somatic embryos developed into plantlets by culturing the mature somatic embryos on 1/2MS medium containing 0.1mg/L IBA.
Eruca sativa Somatic embryogenesis Plant regeneration
ZHANG Tao
College of Life Sciences, ChongQing Normal University, ChongQing, 400047, China;Gansu Academy of Agricultural Science, Lanzhou, 730070, China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
557-559
2007-03-26(万方平台首次上网日期,不代表论文的发表时间)