Characterization of genes of importance in stress resistance in mustard, Brassica juncea
We have been interested in cloning and characterization of genes expressed in mustard in various stress treatments. In this connection, we have characterized two genes, NPR1 and annexin, that respond to various biotic and abiotic stress treatments. The full-length cDNAs of annexins, which encode proteins having molecular masses of 36.2, 36.0, 36.4 and 36.5 KDa (AnnBj1,AnnBj2, AnnBj6, AnnBj7) were isolated from mustard). A partial AnnBj3 was also isolated along with the genomic clones of these annexins. Genomic DNA gel blot analysis indicated that annexins in mustard belonged to a small multigene family. RNA gel blot anlysis showed regulation of annexin transcripts to various signaling molecules or abiotic stresses. Overexpression of AnnBj1 in tobacco conferred tolerance to dehydration, salinity, heavy metal stress and oxidative stresses at various stages. The transgenic plants also showed enhanced resistance to the oomycete pathogen, Phytophthora parasitica var. nicotianae.NPR1 is a gene that has been shown to have a regulatory role in the Systemic Acquired Resistance (SAR) pathway, acting down stream of salicylic acid. We have also cloned and characterized the differential expression of NPR1 in mustard. The behavior of this followed the same pattern that has been elucidated in Arabidopsis showing conservation of SAR pathway in different species. Both these genes appeared to be good candidate genes, and can be deployed in transgenic plants for enhancing their stress tolerance.
Abiotic stress Annexins Oxidative stress NPR1 Systemic acquired resistance (SAR)
Sravan Kumar Jami T.Swathi Anuradha G.Meur Aparna Datta-Gupta S.Prakash P.B.Kirti
School of Life Sciences, University of Hyderabad, Hyderabad-500046, India National Research Centre for Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
596-598
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）