Manipulation of biosynthesis of aliphatic glucosinolates by gene replacement and RNAi gene silencing
Glucosinolates are a large group of plant secondary metabolites found mainly in the order Capparales. Manipulation of biosynthesis of aliphatic glucosinolates was attempted using gene replacement and RNAi gene silencing. Experiments were conducted to replace the functional Alk allele in Brassica rapa with the non functional Alk allele from Brassica oleracea (broccoli)to develop glucoraphanin-rich B. rapa. Gene introgression lines but no gene replacement lines were obtained. Four MAM-like genes involved in glucosinolate biosyntheses, MAM1(At5g23010), MAML(At5g23020), MAML-3 (At1g18500) and MAML-4 (At1g74040) were silenced by RNAi techniques in Arabidopsis and the resulting glucosinolate profiles were studied. All four MAM-like genes, MAM1, MAML, MAML-3 and MAML-4 were shown to be involved in the aliphatic glucosinolate elongation pathway.
Gene replacement MAM-like gene RNAi Alk gene Arabidopsis thaliana Brassica rapa
Zhixia Niu Muqiang Gao Peter B.E.McVetty Fouad Daayf Hongyan Zhu Carlos F.Quiros Genyi Li
Department of Plant Science, University of Manitoba, Winnpeg, MB R3T2N2 Canada Department of Plant and Soil Science, University of Kentucky, KY 40546-0236, USA Department of Plant Sciences, University of California, Davis, CA 95616, USA
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
603-605
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）